Eastern Conference:

  1. Atlanta United FC
  2. Chicago Fire FC
  3. FC Cincinnati
  4. Columbus Crew SC
  5. D.C. United
  6. Inter Miami CF
  7. CF Montréal
  8. Nashville SC
  9. New England Revolution
  10. New York City FC
  11. New York Red Bulls
  12. Orlando City SC
  13. Philadelphia Union
  14. Toronto FC

Western Conference:

  1. Austin FC
  2. Colorado Rapids
  3. FC Dallas
  4. Houston Dynamo FC
  5. LA Galaxy
  6. Los Angeles FC
  7. Minnesota United FC
  8. Portland Timbers
  9. Real Salt Lake
  10. San Jose Earthquakes
  11. Seattle Sounders FC
  12. Sporting Kansas City
  13. Vancouver Whitecaps FC

Expansion teams to be added after 2021 include:

  1. St. Louis City SC (2023)
  2. Sacramento Republic FC (Date uncertain due to ownership issues as of 2021)
  3. Charlotte FC (2022)

Please note that the MLS is continually expanding, and there may be new teams added or team changes.

Teams